The Open Press - Press Release News Wire
  Sunday, October 24th 2021 - 8:37pm EDT
PRESS RELEASE NEWS WIRE         
     FRONT PAGE     |     ARCHIVES     |     ABOUT US     |     FAQS      
Welcome Guest User  ( LOG IN )      SUBMIT A RELEASE   ADD A FIRM   
Sorting Options

By Country

By Metro Area

By Industry

Member Options


Register

Login

Recover Password

Email Alert
Site Navigation

Home

Services & Fees

Press Release Guidelines

Submit a Press Release

Company Directory

Contact Us

RSS Feeds

Help

|Press Releases|Pro PRs|Book Reviews|Articles (OP-ED)|News|Exposé|PR Firms|


Alexander Goryaynov's New Article In Plos One. Role Of Molecular Charge In Nucleocytoplasmic Transport

Submitted by: Temple University

2014-02-26 00:01:13

del.icio.us digg facebook Email twitter print

Alexander Goryaynov and Weidong Yang used super-resolution microscopy approaches to study the role of molecular charge on nucleocytoplasmic transport.

(OPENPRESS) Transport of genetic materials and proteins between the nucleus and cytoplasm of eukaryotic cells is mediated by nuclear pore complexes (NPCs). A selective barrier formed by phenylalanine-glycine (FG) nucleoporins (Nups) with net positive charges in the NPC allows for passive diffusion of signal-independent small molecules and transport-receptor facilitated translocation of signal-dependent cargo molecules. Recently, negative surface charge was postulated to be another essential criterion for selective passage through the NPC. However, the charge-driven mechanism in determining the transport kinetics and spatial transport route for either passive diffusion or facilitated translocation remains obscure. Here we employed high-speed single-molecule fluorescence microscopy with an unprecedented spatiotemporal resolution of 9 nm and 400 ┬Ás to uncover these mechanistic fundamentals for nuclear transport of charged substrates through native NPCs. We found that electrostatic interaction between negative surface charges on transiting molecules and the positively charged FG Nups, although enhancing their probability of binding to the NPC, never plays a dominant role in determining their nuclear transport mode or spatial transport route. A 3D reconstruction of transport routes revealed that small signal-dependent endogenous cargo protein constructs with high positive surface charges that are destined to the nucleus, rather than repelled from the NPC as suggested in previous models, passively diffused through an axial central channel of the NPC in the absence of transport receptors. Finally, we postulated a comprehensive map of interactions between transiting molecules and FG Nups during nucleocytoplasmic transport by combining the effects of molecular size, signal and surface charge.




Contact Info

Temple University
Phone: 7276882067
Website: http://www.alexandergoryaynov.com
1900 North 12th Street



Release Info

Metro Area: ALL REGIONS (Including International) Read More from this Metro Area
Country: United States Read more from this Country
Industry: Science Read more from this Industry
Press Keywords: Alexander,G, Goryaynov, Temple, microscopy, nuclear
Press Company: Temple University
Press Site: http://www.alexandergoryaynov.com




 


Copyright © 2021
The Open Press - Professional Press Release News Wire.
All rights reserved.


   Home|About Us|Archives|Faqs|Services & Fees|Guidelines|Submit Release|Register|Privacy Policy|Contact Us|RSS Feeds|Email Alert